Part:BBa_K678050:Design

pJEJAM2

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2225
  Illegal PstI site found at 2315
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2225
  Illegal PstI site found at 2315
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2225
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2225
  Illegal PstI site found at 2315
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2225
  Illegal PstI site found at 2315
  Illegal NgoMIV site found at 3080
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This plasmid was assembled with the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this plasmid contains linkers between the biological parts as shown on the figure below.

Source

See the individual biobricks